Analysis Note

Positive ControlSV80 or SV-T2 cells

Negative Control3T3 cells

Application

Affinity Purification (use Cat. No. DP02A)r>Immunoblotting (1-5 µg/ml, see application references)r>Immunofluorescence (1-5 µg/ml)r>Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A)r>Paraffin Sections (see application references)

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~94 kDa SV40 large T antigen.

This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) is validated for use in Affinity Purification, Immunoblotting, IF, IP, Paraffin Sections for the detection of SV40 T Antigen (Ab-2).

Recognizes ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells.

SV40 T Ag (Ab-2) is a mouse monoclonal antibody with specificity for antigenic determinants unique to the SV40 large T antigen and non-reactive with SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular transforming protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the p105 product of the retinoblastoma susceptibility gene.

Immunogen

Epitope: Within amino acids 83-128

purified SV40 large T-antigen

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

DeCaprio, J.A., et al. 1988. Cell54, 275.Whyte, P., et al. 1988. Nature334, 124.Mitchell, P.J., et al. 1987. Cell50, 847.Dixon, R.A.F. and Nathans, D., 1985. J. Virol.53, 1001.Simanis, V. and Lane, D.P. 1985. Virol.144, 88. Mann, R.S. and Carroll, R.B. 1984. Virology138, 379. Sarnow, P., et al. 1982. Cell28, 387.Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA78, 41.Lane, D.P. and Crawford, L.V. 1979. Nature278, 261.Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA71, 3754.Tooze, J. 1973. Cold Spring Harbor, New York.Tegtmeyer, P. 1972. J. Virol.10, 591.

The agarose conjugate (Cat. No. DP02A) is also available and suitable for affinity purification and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Packaging

200 µg in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Warning

Toxicity: Standard Handling (A)